The Grant Asymptote EF600 is the world’s first nitrogen and cryogen free controlled rate freezer. It has been specifically designed for use in human and animal IVF applications for the cryopreservation of embryos, stem cells and spermatozoa but can also be used for a wide range of freezing applications.
Unlike conventional controlled rate cooling equipment used in IVF laboratories, the EF600 poses no contamination risk and can be used in cleanrooms. The EF600 fits neatly and quietly on a bench-top and its performance in terms of samples frozen and successfully recovered is proven to be directly comparable with that of a standard liquid nitrogen freezer. Cools down to -100oC.
The cooling rate of the EF600 is precisely controlled, ensuring good recovery of cells upon thawing. Operation is simple and can be carried out with or without a PC to automatically log and display data. Different cooling profiles are available from a drop down menu. Published trials(1) have demonstrated successful freezing and recovery of embryos, sperm and embryonic stem cells.
Please follow this link to download the Asymptote Cool Guide to Cryopreservation by Dr J Morris.
Main applications
The EF600 is versatile equipment which can be used for the cryopreservation of a wide range of samples, including:
- Human and veterinary IVF
- Stem Cells
- Clinical and research samples, e.g. lymphocytes and tissue culture cells in conventional cryovials
- Cord blood in flexible bags
- Microtitre plates - especially for the cryopreservation of adherent cells
- Matrix type tubes – containing cell suspensions which require controlled rates of cooling for recovery on thawing
- May also be integrated into robotic systems, including biorepositories and biobanks
- May also be operated as a portable device: with many veterinary and conservation applications
(1)RBM Online 13, 421-426, 2006; Cryoletters 27(3), 179-184 (2006)